Weiss E S, Ahmed S A, Welch M J, Williamson J R, Ter-Pogossian M M, Sobel B E
Circulation. 1977 Jan;55(1):66-73. doi: 10.1161/01.cir.55.1.66.
To assess myocardial infarction quantitatively in 15 mm thick transverse sections of the canine heart in vivo we utilized a new technique, positron emission transaxial tomography (PETT) and cyclotron-produced 11C-palmitate (11C-P) injected intravenously. Results were compared to regional myocardial creatine phosphokinase (CPK) depletion, diminished 14C-palmitate accumulation in tissue extracts, and infarction estimated morphometrically 48 hours after coronary occlusion. CPK activity and 14C-P content declined in parallel in transmural biopsies (N=44) from normal and ischemic zones (r=.92) in six dogs; and infarct in 10 mm thick cross sections of the entire left ventricle estimated morphometrically (N=26) in six other animals correlated with CPK depletion in contiguous 2.5 mm thick slices (r=.92). When the percentage of infarction in 15 mm thick cross sections was assessed tomographically in six other dogs 48 hours after coronary occlusion with 11C-P injected intravenously, results correlated with infarction in corresponding cross sections from the same hearts estimated morphometrically (r=.97, N=9) and by analysis of CPK depletion (r=.93, N=9). Thus, PETT permits estimation of infarction in cross sections of the left ventricle in vivo after intravenous injection of 11C-palmitate.
为了在活体犬心脏15毫米厚的横断面上定量评估心肌梗死情况，我们采用了一种新技术——正电子发射断层扫描（PETT），并静脉注射了回旋加速器产生的11C-棕榈酸盐（11C-P）。将结果与区域心肌肌酸磷酸激酶（CPK）耗竭、组织提取物中14C-棕榈酸盐积累减少以及冠状动脉闭塞48小时后通过形态测量法估计的梗死情况进行比较。在六只犬的正常和缺血区域的透壁活检组织（N = 44）中，CPK活性和14C-P含量平行下降（r = 0.92）；在另外六只动物中，通过形态测量法估计整个左心室10毫米厚横断面上的梗死情况（N = 26）与相邻2.5毫米厚切片中的CPK耗竭相关（r = 0.92）。在另外六只犬冠状动脉闭塞48小时后，静脉注射11C-P，通过断层扫描评估15毫米厚横断面上的梗死百分比，结果与同一心脏相应横断面上通过形态测量法估计的梗死情况（r = 0.97，N = 9）以及通过CPK耗竭分析得出的结果（r = 0.93，N = 9）相关。因此，静脉注射11C-棕榈酸盐后，PETT能够在活体中估计左心室横断面上的梗死情况。
