[Metal resistance of gram-negative bacteria isolated from soil and waste waters of industrial regions].

Metals resistance of 112 strains of gram-negative bacteria isolated from soil and wastes around Nizniy Novgorod plants were investigated. MIC of metals varied in range between 1-10 mM Ni2+, Co2+, 1-6 mM Zn2+, 1-4 mM Cd2+, CrO4(2-), and 0.1-0.3 mM TeO3(2-). Bacteria with phenotypes NiCo and NiZnTe are revealed most frequent (25.6 and 6.4% accordingly). Plasmid DNAs with size between 2-70 kbp were found in 50% isolated strains. It was shown that resistance to cobalt of unidentified gram-negative bacterium is determined by plasmid genes. Recombinant plasmid contained fragment DNA controlling resistance to cadmium and zinc from Pseudomonas spp. strain was constructed by using helper plasmid pULB113.