Abo-Amer Aly E, Mohamed Rehab M
Division of Microbiology, Department of Botany, Faculty of Science, Sohag University, Sohag, Egypt.
Roum Arch Microbiol Immunol. 2006 Jul-Dec;65(3-4):113-9.
Solar-degrading bacteria, Pseudomonas aeruginosa strains, were isolated from Egyptian soil by Mineral Salt Medium (MSM) supplemented with Solar (motor fuel) from different oil-contaminated sites in Sohag province. The strain AA301 of Pseudomonas aeruginosa showed appreciable growth in MSM medium containing high concentrations of Solar ranging from 0.5 to 3% (v/v), with optimum concentration at 1.5%. Solar was used as a sole carbon source and a source of energy by the bacterium. The ability to degrade Solar was found to be associated with a single 60-kb plasmid designated pSOL15. The plasmid-cured variant, which was obtained by culturing in LB broth with kanamycin, lost the plasmid indicative the ability to degrade Solar must depend on this plasmid. The wild type isolate, Pseudomonas aeruginosa AA301 and transformant strain, have maximum growth (OD600 = approximately 2) on Solar, however the plasmid-cured variant did not have any significant growth on Solar. Moreover, resistance to a wide range of heavy metals such as Mn2+, Hg2+, Mg2+, Cd2+, Zn2+, and Ni2+ was also 60-kb plasmid-mediated. Therefore, the strain AA301 could be good candidate for remediation of some heavy metals and oil hydrocarbons in heavily polluted sites.
从埃及土壤中分离出可降解太阳能的细菌——铜绿假单胞菌菌株，采用添加了来自索哈杰省不同石油污染场地的太阳能（车用燃料）的无机盐培养基（MSM）进行分离。铜绿假单胞菌AA301菌株在含有0.5%至3%（v/v）高浓度太阳能的MSM培养基中生长良好，最适浓度为1.5%。太阳能被该细菌用作唯一碳源和能源。发现降解太阳能的能力与一个名为pSOL15的60kb质粒有关。通过在含有卡那霉素的LB肉汤中培养获得的质粒消除变体失去了该质粒，这表明降解太阳能的能力一定依赖于该质粒。野生型菌株铜绿假单胞菌AA301和转化菌株在太阳能上生长最佳（OD600约为2），然而质粒消除变体在太阳能上没有明显生长。此外，对多种重金属如Mn2+、Hg2+、Mg2+、Cd2+、Zn2+和Ni2+的抗性也是由60kb质粒介导的。因此，AA301菌株可能是重度污染场地中某些重金属和石油烃修复的良好候选菌株。
