Sureau A, Perbal B
Laboratoire d'Oncologie Virale et Moléculaire, Institut Curie, Orsay, France.
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):932-6. doi: 10.1073/pnas.91.3.932.
We have recently established that several mRNAs encode the PR264/SC35 splicing factor and that their expression is related to that of c-Myb in human hematopoietic cells. We now report that the various PR264 mRNAs whose expression is transactivated by c-Myb proteins encode an identical PR264/SC35 polypeptide. These mRNAs differ only in their 3' untranslated sequences and exhibit significantly different half-lives. We also show that there is a direct correlation between the length of the 3' untranslated region and the stability of the mRNA species. Given that 5' and 3' splice site selection mediated by the PR264/SC35 splicing factor is concentration-dependent, we postulate that alternative splicing of the 3' untranslated sequences might represent another key element in regulating the levels of PR264/SC35 expression during hematopoietic differentiation.
我们最近证实，有几种mRNA编码PR264/SC35剪接因子，并且它们的表达与人类造血细胞中c-Myb的表达相关。我们现在报告，其表达被c-Myb蛋白反式激活的各种PR264 mRNA编码相同的PR264/SC35多肽。这些mRNA仅在其3'非翻译序列上有所不同，并且表现出明显不同的半衰期。我们还表明，3'非翻译区的长度与mRNA种类的稳定性之间存在直接相关性。鉴于由PR264/SC35剪接因子介导的5'和3'剪接位点选择是浓度依赖性的，我们推测3'非翻译序列的可变剪接可能代表造血分化过程中调节PR264/SC35表达水平的另一个关键因素。
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