腐蚀艾肯菌遗传系统的开发：质粒pFM739和pLES2的转移

Development of a genetic system for Eikenella corrodens: transfer of plasmids pFM739 and pLES2.

作者信息

Rao V K, Whitlock J A, Progulske-Fox A

机构信息

Department of Oral Biology, University of Florida, Gainesville 32610-0424.

出版信息

Plasmid. 1993 Nov;30(3):289-95. doi: 10.1006/plas.1993.1062.

DOI:10.1006/plas.1993.1062

PMID:8302938

Abstract

Eikenella corrodens is a Gram-negative microaerophilic rod which is emerging as an important human pathogen. Elucidation of the mechanisms by which it causes disease require efficient methods for the transfer of DNA to E. corrodens. Plasmids pFM739 and pLES2 have been transferred by conjugation from Escherichia coli S17-1 to E. corrodens ATCC 23834 at frequencies of 2.5 x 10(-7) and 2.42 x 10(-7), respectively. In addition, both plasmids could be transferred to four additional, clinical strains of E. corrodens at a similar frequency. The use of bacteriophage T4 as a counterselecting agent is also described.

摘要

腐蚀埃肯菌是一种革兰氏阴性微需氧杆菌，正逐渐成为一种重要的人类病原体。阐明其致病机制需要有效的将DNA转移至腐蚀埃肯菌的方法。质粒pFM739和pLES2已通过接合作用从大肠杆菌S17-1转移至腐蚀埃肯菌ATCC 23834，转移频率分别为2.5×10⁻⁷和2.42×10⁻⁷。此外，这两种质粒还能以相似的频率转移至另外四株临床分离的腐蚀埃肯菌菌株。本文还描述了使用噬菌体T4作为反选择剂的情况。