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通过任意引物PCR检测个体受试者口腔腐蚀艾肯菌的克隆多样性。

Clonal diversity of oral Eikenella corrodens within individual subjects by arbitrarily primed PCR.

作者信息

Chen C, Ashimoto A

机构信息

University of Southern California School of Dentistry, Department of Periodontology, Los Angeles 90089, USA.

出版信息

J Clin Microbiol. 1996 Jul;34(7):1837-9. doi: 10.1128/JCM.34.7.1837-1839.1996.

Abstract

The genetic diversity of 205 Eikenella corrodens isolates recovered from dental plaque, mucosal surfaces, and saliva of 24 subjects was examined by arbitrarily primed PCR. Twenty-two subjects were colonized by multiple clones (range, two to eight; mean, 3.7). This study demonstrates the utility of arbitrarily primed PCR for clonal analysis of E. corrodens and the multiclonal colonization of E. corrodens in the oral cavity.

摘要

通过任意引物PCR检测了从24名受试者的牙菌斑、黏膜表面和唾液中分离出的205株啮蚀艾肯菌的遗传多样性。22名受试者被多个克隆定殖(范围为2至8个;平均为3.7个)。本研究证明了任意引物PCR在啮蚀艾肯菌克隆分析中的实用性以及该菌在口腔中的多克隆定殖情况。

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