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通过任意引物PCR检测个体受试者口腔腐蚀艾肯菌的克隆多样性。

Clonal diversity of oral Eikenella corrodens within individual subjects by arbitrarily primed PCR.

作者信息

Chen C, Ashimoto A

机构信息

University of Southern California School of Dentistry, Department of Periodontology, Los Angeles 90089, USA.

出版信息

J Clin Microbiol. 1996 Jul;34(7):1837-9. doi: 10.1128/JCM.34.7.1837-1839.1996.

DOI:10.1128/JCM.34.7.1837-1839.1996
PMID:8784606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229131/
Abstract

The genetic diversity of 205 Eikenella corrodens isolates recovered from dental plaque, mucosal surfaces, and saliva of 24 subjects was examined by arbitrarily primed PCR. Twenty-two subjects were colonized by multiple clones (range, two to eight; mean, 3.7). This study demonstrates the utility of arbitrarily primed PCR for clonal analysis of E. corrodens and the multiclonal colonization of E. corrodens in the oral cavity.

摘要

通过任意引物PCR检测了从24名受试者的牙菌斑、黏膜表面和唾液中分离出的205株啮蚀艾肯菌的遗传多样性。22名受试者被多个克隆定殖(范围为2至8个;平均为3.7个)。本研究证明了任意引物PCR在啮蚀艾肯菌克隆分析中的实用性以及该菌在口腔中的多克隆定殖情况。

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本文引用的文献

1
Comparison of arbitrarily primed polymerase chain reaction and ribotyping for subtyping Actinobacillus actinomycetemcomitans.任意引物聚合酶链反应与核糖体分型用于伴放线放线杆菌分型的比较
Anaerobe. 1995 Apr;1(2):97-102. doi: 10.1006/anae.1995.1004.
2
Clonal analysis of Porphyromonas gingivalis by the arbitrarily primed polymerase chain reaction.利用任意引物聚合酶链反应对牙龈卟啉单胞菌进行克隆分析。
Oral Microbiol Immunol. 1994 Apr;9(2):99-103. doi: 10.1111/j.1399-302x.1994.tb00042.x.
3
Evaluating two methods for fingerprinting genomes of Actinobacillus actinomycetemcomitans.评估两种用于放线共生放线杆菌基因组指纹图谱分析的方法。
Oral Microbiol Immunol. 1993 Dec;8(6):337-43. doi: 10.1111/j.1399-302x.1993.tb00608.x.
4
Eikenella corrodens in the human oral cavity.人类口腔中的啮蚀艾肯菌。
J Periodontol. 1989 Nov;60(11):611-6. doi: 10.1902/jop.1989.60.11.611.
5
Outer membrane protein and lipopolysaccharide heterogeneity among Eikenella corrodens isolates.
J Infect Dis. 1990 Sep;162(3):664-71. doi: 10.1093/infdis/162.3.664.
6
Restriction endonuclease analysis of Eikenella corrodens.腐蚀艾肯菌的限制性内切酶分析
J Clin Microbiol. 1990 Jun;28(6):1265-70. doi: 10.1128/jcm.28.6.1265-1270.1990.
7
Eikenella corrodens in human oral and non-oral infections: a review.人类口腔及非口腔感染中的腐蚀艾肯菌:综述
J Periodontol. 1992 Dec;63(12):941-53. doi: 10.1902/jop.1992.63.12.941.