Yun Y S, Lee Y S, Jo S K, Jung I S
Laboratory of Immunology, Korea Cancer Center Hospital, KAERI, Seoul.
Planta Med. 1993 Dec;59(6):521-4. doi: 10.1055/s-2006-959752.
The water extract of Panax ginseng was fractionated by its solubility in ethanol and then the ethanol-insoluble fraction was tested for immunomodulatory activity. The ethanol-insoluble fraction of ginseng (Fr. 3) proliferated splenocytes and generated activated killer cells in vitro. These activated killer cells killed both NK cell sensitive and insensitive tumor target cells without MHC-restriction. Activation of splenocytes by ginseng was mediated through the endogenously produced IL-2. To investigate the effects of Fr.3 on the autochthonous neoplasm, a single subcutaneous injection of 0.5 mg of benzo[a]pyrene (BP) was given within 24 hours after birth of male N: GP(S) mice, and Fr.3 was administered in drinking water at a concentration of 2 mg/ml, 1 mg/ml, or 0.5 mg/ml for 6 weeks after weaning. The treatment with Fr. 3 significantly inhibited lung tumor incidence (P < 0.05) compared with the BP alone group at a concentration of 2 mg/ml or 1 mg/ml in drinking water at the 9th week after BP treatment. These results suggest that the ethanol-insoluble fraction of ginseng shows antitumor effects as an immunomodulator.
人参水提取物按其在乙醇中的溶解度进行分级分离，然后对乙醇不溶级分进行免疫调节活性测试。人参的乙醇不溶级分（级分3）在体外可使脾细胞增殖并产生活化杀伤细胞。这些活化杀伤细胞可杀伤NK细胞敏感和不敏感的肿瘤靶细胞，且不受MHC限制。人参对脾细胞的激活是通过内源性产生的白细胞介素-2介导的。为研究级分3对自发肿瘤的影响，在雄性N: GP(S)小鼠出生后24小时内单次皮下注射0.5毫克苯并[a]芘（BP），断奶后以2毫克/毫升、1毫克/毫升或0.5毫克/毫升的浓度在饮用水中给予级分3，持续6周。在BP处理后第9周，与仅给予BP的组相比，饮用水中级分3浓度为2毫克/毫升或1毫克/毫升时，级分3处理显著抑制了肺肿瘤发生率（P <0.05）。这些结果表明，人参的乙醇不溶级分作为一种免疫调节剂具有抗肿瘤作用。
