Cunha R G, Passos W S, Souza M C
Departamento de Patologia e Clínica, Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, RJ.
Rev Bras Biol. 1993 Feb;53(1):29-36.
In the present paper the pathogenicity of equine subtype A/equi 1 (H7N7) and A/equi 2 (H3N8) for chicks was studied. Strains previously isolated in Brazil, representatives of both subtypes, were used. Eight experiments were performed for A/equi 2, using 89 chicks (4 to 18-day old). Six hundred thirty three samples of cloacal material were collected from 01 to 15 days pos-infection (p.i.) and inoculated in 11-day old chick embryos for recuperation of virus. Twelve samples showed positive results. The recuperated viruses were identified with specific antiserum in hemagglutination inhibition test (HI). Blood samples of all chicks collected prior to infection showed no antibodies to both subtypes. Chicks inoculated with A/equi 2 virus were bled 18 to 21 days p.i. Out of 89, seventy one (79.8%) serums showed different levels of antibodies at HI tests. Seventy chicks were inoculated with A/equi 1 subtype. Five hundred forty three samples of cloacal material were harvested and inoculated in embryonated chick eggs. No recuperation of virus occurred. However, all the inoculated chickens showed seroconversion. Chicks infected with A/equi 2 may shed virus in feces. No signs of disease were noted in the inoculated chicks.
在本论文中,研究了马A/equi 1(H7N7)和A/equi 2(H3N8)亚型对雏鸡的致病性。使用了先前在巴西分离的两种亚型的代表毒株。对A/equi 2进行了8次实验,使用了89只雏鸡(4至18日龄)。在感染后第1至15天(p.i.)从泄殖腔采集了633份样本,并接种到11日龄鸡胚中以回收病毒。12份样本呈阳性结果。回收的病毒在血凝抑制试验(HI)中用特异性抗血清进行鉴定。在感染前采集的所有雏鸡的血液样本对两种亚型均未显示抗体。接种A/equi 2病毒的雏鸡在感染后18至21天采血。89只中有71只(79.8%)血清在HI试验中显示出不同水平的抗体。70只雏鸡接种了A/equi 1亚型。采集了543份泄殖腔样本并接种到鸡胚中。未回收病毒。然而,所有接种的鸡均出现血清转化。感染A/equi 2的雏鸡可能通过粪便排出病毒。在接种的雏鸡中未观察到疾病迹象。