[通过竞争性斑点杂交法测定甲型流感病毒实验室重配株的基因型]
[The determination of the genotype of laboratory reassortants of the influenza A virus by competitive dot hybridization].
作者信息
Golubev D B, Zolotarev F N, Iukhnova L G
出版信息
Vopr Virusol. 1993 Nov-Dec;38(6):261-3.
PMID:8303886
Abstract
The genotype of laboratory reassortants produced by co-cultivation of virulent A/USSR/2/85 (H3N2) virus and multi-passaged attenuation donor A/Leningrad/9/46 virus (H1N1) was determined by two different methods: a method of competitive dot hybridization (CDH) and polyacrylamide gel electrophoresis (PAGE) with virion RNAs urea. The results of the two methods were shown to be completely identical which confirmed the potential of using CDH method for genetic typing of influenza A virus reassortant strains.
摘要
通过两种不同方法确定了由强毒株A/USSR/2/85（H3N2）病毒与多代传代减毒株供体A/列宁格勒/9/46病毒（H1N1）共同培养产生的实验室重配体的基因型：竞争点杂交（CDH）法和病毒粒子RNA尿素聚丙烯酰胺凝胶电泳（PAGE）法。两种方法的结果完全相同，这证实了使用CDH法对甲型流感病毒重配毒株进行基因分型的潜力。
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