Haginaka J, Nishimura Y, Yasuda H
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
J Pharm Biomed Anal. 1993 Oct;11(10):1023-6. doi: 10.1016/0731-7085(93)80064-8.
A high-performance liquid chromatographic method has been developed for the determination of cyclodextrins (CDs) in serum. The method involves solid-phase extraction of CDs, separation on a C18 reversed-phase column using a mixture of water, tetrahydrofuran and methanol as an eluent, eluent pH modification with a cation-exchange membrane reactor surrounded by 1.5 M sodium hydroxide solutions, and pulsed amperometric detection (PAD) with a gold working electrode. The solid-phase extraction on a C18 bonded-silica column was effective for removing the PAD sensitive components in serum. The calibration graphs constructed by internal standard method were linear over the range 6.25-200 pmol of CDs in serum. The detection limits for CDs were about 5 pmol at a signal-to-noise ratio of 3.
已开发出一种高效液相色谱法用于测定血清中的环糊精（CDs）。该方法包括环糊精的固相萃取，使用水、四氢呋喃和甲醇的混合物作为洗脱剂在C18反相柱上进行分离，通过被1.5 M氢氧化钠溶液包围的阳离子交换膜反应器对洗脱剂pH进行调节，以及使用金工作电极进行脉冲安培检测（PAD）。在C18键合硅胶柱上的固相萃取有效地去除了血清中对PAD敏感的成分。通过内标法构建的校准曲线在血清中环糊精含量为6.25 - 200 pmol范围内呈线性。环糊精的检测限在信噪比为3时约为5 pmol。
