Haginaka J, Nishimura Y, Wakai J, Yasuda H, Koizumi K, Nomura T
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Anal Biochem. 1989 Jun;179(2):336-40. doi: 10.1016/0003-2697(89)90139-5.
A high-performance liquid chromatographic method has been developed for the determination of cyclodextrins (CDs) and branched CDs. The method involves their separation on a reversed-phase column using a mixture of water and acetonitrile as an eluant, eluant pH modification with a cation-exchange membrane reactor surrounded by 1.5 M sodium hydroxide solutions, and pulsed amperometric detection with a gold working electrode. The calibration graphs constructed by peak height versus injected amount were linear over the ranges 50-1000 pmol. The detection limits for CDs and branched CDs were about 1-5 pmol at a signal-to-noise ratio of 3. The method was successfully applied to the assay of beta-CD in serum samples.
已开发出一种高效液相色谱法用于测定环糊精（CDs）和支链环糊精。该方法包括在反相柱上使用水和乙腈的混合物作为洗脱剂进行分离，通过被1.5 M氢氧化钠溶液包围的阳离子交换膜反应器调节洗脱剂的pH值，以及使用金工作电极进行脉冲安培检测。通过峰高与进样量构建的校准曲线在50 - 1000 pmol范围内呈线性。在信噪比为3时，CDs和支链环糊精的检测限约为1 - 5 pmol。该方法已成功应用于血清样品中β-环糊精的测定。
