Braselton W E, Bransome E D, Huff T A
Diabetes. 1977 Jan;26(1):50-7. doi: 10.2337/diab.26.1.50.
A method is described for measurement of chlorpropamide, tolbutamide, and the tolbutamide metabolites hydroxymethyltolbutamide and carbotytolbutamide in blood. It consists of formation of the thermally stable methyl-trifluoroacetyl derivatives of chlorpropamide and tolbutamide, and the methyl-heptafluorobutyryl derivatives of hydroxymethyltolbutamide and carboxytolbutamide, which are then analyzed by electron-capture gas chromatography. Measurements of blood levels of the compounds with this method have been verified by quantitation of the same samples using gas chromatography-mass spectrometry in the mass fragmentography mode. Blood level values and beta-phase half-time disappearance rates of chlorpropamide, tolbutamide, hydroxymethyltolbutamide, and carboxytolbutamide were measured in normal volunteers following an oral dose of chlorpropamide or tolbutamide. Blood levels of the four compounds were also determined in a few diabetics receiving continuous daily treatment.
本文描述了一种测定血液中氯磺丙脲、甲苯磺丁脲及其代谢产物羟甲基甲苯磺丁脲和羧基甲苯磺丁脲的方法。该方法包括将氯磺丙脲和甲苯磺丁脲转化为热稳定的甲基 - 三氟乙酰衍生物，以及将羟甲基甲苯磺丁脲和羧基甲苯磺丁脲转化为甲基 - 七氟丁酰衍生物，然后通过电子捕获气相色谱法进行分析。使用该方法测定的化合物血液水平值已通过在质量碎片图模式下使用气相色谱 - 质谱法对相同样品进行定量分析得到验证。在正常志愿者口服氯磺丙脲或甲苯磺丁脲后，测定了氯磺丙脲、甲苯磺丁脲、羟甲基甲苯磺丁脲和羧基甲苯磺丁脲的血液水平值及β相半衰期消失率。还对一些接受每日持续治疗的糖尿病患者测定了这四种化合物的血液水平。
