Teerlink T, Hennekes M W, van Leeuwen P A, Houdijk A
Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
Clin Chim Acta. 1993 Sep 30;218(2):159-68. doi: 10.1016/0009-8981(93)90180-c.
A high-performance liquid chromatographic method for the determination of glutamine in biological samples is presented. Glutamine was derivatized with ortho-phthalaldehyde reagent containing 3-mercaptopropionic acid and separated by reversed phase chromatography on a C18 column containing 3-microns particles. No interference from other amino acids was observed. The assay was linear over a range from 1 to 2,000 mumol/l. Analytical recovery of plasma samples spiked with glutamine was 98.6 +/- 3.8%. Within- and between-batch imprecision were 1.5% and 2.2%, respectively. The derivatization step was fully automated. Total analysis time, including derivatization and chromatography, amounted to 6 min. The method can be used for the determination of glutamine in plasma, urine, cerebrospinal fluid and tissue homogenates.
本文介绍了一种用于测定生物样品中谷氨酰胺的高效液相色谱法。谷氨酰胺用含3-巯基丙酸的邻苯二甲醛试剂衍生化，然后在含有3微米颗粒的C18柱上进行反相色谱分离。未观察到其他氨基酸的干扰。该测定在1至2000μmol/L范围内呈线性。加标谷氨酰胺的血浆样品的分析回收率为98.6±3.8%。批内和批间不精密度分别为1.5%和2.2%。衍生化步骤完全自动化。包括衍生化和色谱分析在内的总分析时间为6分钟。该方法可用于测定血浆、尿液、脑脊液和组织匀浆中的谷氨酰胺。
