Oster G, Oster S, Havran R T
Acta Cytol. 1978 Jul-Aug;22(4):234-8.
A cytologic staining technique in which the reaction produces an insoluble colored precipitate at the site of trypsin-like proteolysis was applied to human vaginal smears, taken daily over six menstrual cycles. The day of urinary LH surge for each cycle was determined. The enzyme action, namely hydrolysis by plasminogen activator, is confined mainly to intermediate squamous cells. The number of cells stained by the enzyme method reaches a maximum three days prior to the LH surge, whereas the maximum in karyopyknosis occurs close to or at the day of LH surge. The enzyme staining method can thus be used when anticipation of ovulation by about 4 days is needed. No immediate fixation is required prior to the enzyme staining.
一种细胞染色技术被应用于人类阴道涂片，该技术中反应会在类胰蛋白酶样蛋白水解部位产生不溶性有色沉淀，这些涂片取自六个月经周期内的每日样本。确定了每个周期中尿促黄体生成素（LH）激增的日期。酶的作用，即纤溶酶原激活剂的水解作用，主要局限于中层鳞状细胞。酶法染色的细胞数量在LH激增前三天达到最大值，而核固缩的最大值出现在LH激增日或接近LH激增日。因此，当需要提前约4天预测排卵时，可以使用酶染色法。酶染色前无需立即固定。
