Van der Auwera G, Chapelle S, De Wachter R
Universiteit Antwerpen, Departement Biochemie, Belgium.
FEBS Lett. 1994 Jan 31;338(2):133-6. doi: 10.1016/0014-5793(94)80350-1.
The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and ciliates, a cluster which seems related to the fungi. In the course of this work, we developed a set of primers which allow sequencing and PCR amplification of eukaryotic large ribosomal subunit RNA genes of a wide range of phylogenetically distant organisms.
为了研究卵菌纲大雄疫霉5.8S和28S核糖体RNA（rRNA）序列的二级结构，并评估卵菌纲在真核生物中的系统发育地位，我们测定了这些序列。初步结果表明卵菌纲、双鞭毛虫和纤毛虫之间存在联系，这一类群似乎与真菌有关。在这项研究过程中，我们开发了一套引物，可用于对多种系统发育关系较远的生物的真核生物大核糖体亚基RNA基因进行测序和聚合酶链反应（PCR）扩增。
