Motoyama J, Eto K
Department of Developmental Biology, Graduate School of Dentistry, Tokyo Medical and Dental University, Japan.
FEBS Lett. 1994 Feb 7;338(3):319-22. doi: 10.1016/0014-5793(94)80291-2.
Retinoic acid receptor (RAR) gamma gene is expressed in the precartilaginous cells during chondrogenesis in mouse embryos, but the role of the gene products is still unclear. To examine the role during chondrogenesis, we isolated mesenchymal cells from the limb bud of mouse embryos and exposed them to antisense RAR gamma-1 oligodeoxynucleotide in micromass culture. The antisense oligodeoxynucleotide inhibited RAR gamma-1 protein expression and enhanced chondrogenesis in the exposed cells. These results suggest that the complex of RAR gamma-1 protein and its ligand RA acts as a suppressor of the chondrogenesis in the limb development.
维甲酸受体（RAR）γ基因在小鼠胚胎软骨形成过程中的软骨前体细胞中表达，但其基因产物的作用仍不清楚。为了研究其在软骨形成过程中的作用，我们从小鼠胚胎的肢芽中分离间充质细胞，并在微团培养中将它们暴露于反义RARγ-1寡脱氧核苷酸。反义寡脱氧核苷酸抑制了RARγ-1蛋白的表达，并增强了暴露细胞中的软骨形成。这些结果表明，RARγ-1蛋白及其配体RA的复合物在肢体发育中作为软骨形成的抑制剂起作用。
