Motoyama J, Eto K
Department of Developmental Biology, Graduate School of Dentistry, Tokyo Medical and Dental University, Japan.
FEBS Lett. 1994 Feb 7;338(3):323-5. doi: 10.1016/0014-5793(94)80292-0.
To examine the role of c-myc protein during chondrogenesis, we exposed 11 day p.c. mouse limb mesenchymal cells to the antisense c-myc oligonucleotide in micromass culture. The antisense oligonucleotide inhibited the c-myc protein expression, and intensely promoted chondrogenesis in the exposed cells. Most of the cells differentiated into cartilaginous cells, whereas they differentiated into cartilaginous and fibrous cells under the control conditions. The antisense oligonucleotide increased the inhibitory efficiency of all-trans retinoic acid (RA) to the chondrogenesis. These results suggest that the c-myc protein suppress the chondrogenesis and reduces RA responsiveness in the limb mesenchymal cells.
为研究c-myc蛋白在软骨形成过程中的作用，我们将妊娠11天小鼠的肢体间充质细胞在微团培养中暴露于反义c-myc寡核苷酸。反义寡核苷酸抑制了c-myc蛋白的表达，并强烈促进了暴露细胞的软骨形成。大多数细胞分化为软骨细胞，而在对照条件下它们分化为软骨细胞和纤维细胞。反义寡核苷酸提高了全反式维甲酸（RA）对软骨形成的抑制效率。这些结果表明，c-myc蛋白抑制肢体间充质细胞的软骨形成并降低RA反应性。
