Cherkin A, Exkardt M J
J Gerontol. 1977 Jan;32(1):38-45. doi: 10.1093/geronj/32.1.38.
The lysosome hypothesis of aging predicts that membrane stabilizers will extend life-span. Stabilizers containing the dimethylaminoethanol moiety (DMAE) have been reported to extend the life-span of drosophila and mice. We tested the prediction in Japanese quail (N = 15) by administering DMAE bitartrate (18.4 mg/kg/day) in the drinking water for 69 weeks, starting at 195 weeks of age. A matched control group (N = 14) received tartaric acid (4.0 mg/kg/day) in the water. Contrary to the prediction, the DMAE-treated group has a shorter life-span after start of treatment (49 weeks) than the controls (69 weeks). No significant differences between the groups were observed in body weight or daily fluid intake. Three behavioral studies were carried out on survivors at 243-249 weeks of age, namely; activity response to light-flash; sexual mounting response to a female quail; and classical conditioning of the heart rate. Aged quail differed from young-adults in changes in motor activity in response to light flashes. Aged quail appeared less responsive initially to reinforced conditioning trials and demonstrated extinction when light flash was not followed by electric shock. There were no detectable differences in latency to mount or in basal heart rate, either as a function of age or as a function of DMAE treatment.
衰老的溶酶体假说预测，膜稳定剂将延长寿命。据报道，含有二甲基氨基乙醇部分（DMAE）的稳定剂可延长果蝇和小鼠的寿命。我们以日本鹌鹑（N = 15）为实验对象进行验证，从195周龄开始，在饮水中给予酒石酸氢胆碱（18.4毫克/千克/天），持续69周。一个匹配的对照组（N = 14）在水中接受酒石酸（4.0毫克/千克/天）。与预测相反，经DMAE处理的组在开始治疗后（49周）的寿命比对照组（69周）短。两组在体重或每日液体摄入量方面未观察到显著差异。对243 - 249周龄的存活鹌鹑进行了三项行为研究，即：对闪光的活动反应；对雌性鹌鹑的性爬跨反应；以及心率的经典条件反射。老龄鹌鹑与年轻鹌鹑在对闪光的运动活动变化方面存在差异。老龄鹌鹑最初对强化条件反射试验的反应似乎较小，并且当闪光后不伴有电击时会出现消退现象。在爬跨潜伏期或基础心率方面，无论是作为年龄的函数还是作为DMAE处理的函数，均未检测到差异。
