尸检标本中Lewis血型抗原及分泌状态的评估
Assessment of Lewis blood group antigens and secretor status in autopsy specimens.
作者信息
Busuttil A, Blackwell C C, James V S, MacKenzie D A, Saadi A T, Weir D M
机构信息
Department of Forensic Medicine, Medical School, University of Edinburgh, Scotland, UK.
出版信息
Forensic Sci Int. 1993 Oct;61(2-3):133-40. doi: 10.1016/0379-0738(93)90221-u.
The ability of enzyme linked immunosorbent assays (ELISA) to detect Lewis and H antigens in secretions obtained from 280 autopsies was assessed. The ELISA results were compared with those for matched blood specimens examined by agglutination of erythrocytes by monoclonal anti-Lewis(a) and anti-Lewis(b) antibodies. There was good agreement between the results for the two tests and the ELISAs could be used to determine secretor status of the subject. While determination of ABO group with monoclonal anti-A and anti-B was possible even with lysed blood, the results for Lewis typing by erythrocyte agglutination were poor if the sample was lysed or partially lysed. Detection of the antigens by ELISA was as efficient among elderly subjects as among younger ones and both H and Lewis antigens could be detected on erythrocytes and in secretions up to 127 h after death.
评估了酶联免疫吸附测定（ELISA）检测280例尸检获得的分泌物中Lewis和H抗原的能力。将ELISA结果与通过单克隆抗Lewis(a)和抗Lewis(b)抗体凝集红细胞检测的匹配血液标本的结果进行比较。两种检测结果之间具有良好的一致性，ELISA可用于确定受试者的分泌型状态。虽然使用单克隆抗A和抗B抗体即使对溶血的血液也能确定ABO血型，但如果样本溶血或部分溶血，通过红细胞凝集进行Lewis分型的结果较差。ELISA检测抗原在老年受试者中与年轻受试者中一样有效，并且在死后长达127小时的红细胞和分泌物中都能检测到H和Lewis抗原。
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