Orkin R W, Gehron P, McGoodwin E B, Martin G R, Valentine T, Swarm R
J Exp Med. 1977 Jan 1;145(1):204-20. doi: 10.1084/jem.145.1.204.
We have studied a murine tumor previously classified as a poorly differentiated chondrosarcoma. Although the cells in this tumor are surrounded by large quantities of extracellular matrix material, the matrix fails to react with stains specific for the sulfated glycosaminoglycans present in normal cartilage. Here we show at the ultrastructural level that the tumor matrix is a homogeneous, nonfibrillar material, resembling basement membrane. Neither the proteoglycan matrix granules nor collagen fibrils characteristic of cartilage are present in the tumor matrix. Amino acid analyses of whole tumor tissue, enzyme-solubilized tumor components, and the protein extracted from lathyritic tumors confirmed that the tumor matrix is a basement membrane collagen. The collagenous protein extracted from the tumor by nonenzymatic means contains three unique polypeptides larger than the alpha-chain components of the other types of collagen. These studies indicate that the tumor is not a type of chondrosarcoma but a basement membrane producing tumor.
我们研究了一种先前被归类为低分化软骨肉瘤的小鼠肿瘤。尽管该肿瘤中的细胞被大量细胞外基质物质所包围，但该基质不能与正常软骨中存在的硫酸化糖胺聚糖的特异性染色剂发生反应。在此我们在超微结构水平上显示，肿瘤基质是一种均匀的、无纤维的物质，类似于基底膜。肿瘤基质中既不存在软骨特有的蛋白聚糖基质颗粒，也不存在胶原纤维。对整个肿瘤组织、酶溶性肿瘤成分以及从患骨生成障碍病的肿瘤中提取的蛋白质进行的氨基酸分析证实，肿瘤基质是一种基底膜胶原蛋白。通过非酶法从肿瘤中提取的胶原蛋白质含有三种独特的多肽，其分子量大于其他类型胶原的α链成分。这些研究表明，该肿瘤不是软骨肉瘤的一种类型，而是一种产生基底膜的肿瘤。
