Stansbridge E M, Mills G A, Walker V
Southampton University Medical School, Southampton General Hospital, UK.
J Chromatogr. 1993 Nov 17;621(1):7-13. doi: 10.1016/0378-4347(93)80070-k.
A method was developed and validated for analysis of faecal short-chain fatty acids using automated headspace gas chromatography. Quantification was by standard addition. Ghosting was minimized by lining the transfer tube from the headspace sampler to the gas chromatograph with deactivated fused silica and addition of formic acid to sample vials. Saturation of samples with lithium sulphate increased recoveries. The method was used to analyse small amounts of faecal matter collected from premature babies. Advantages of the technique are rapid, accurate, analysis of faecal specimens in batches, with minimum sample preparation.
开发并验证了一种使用自动顶空气相色谱法分析粪便短链脂肪酸的方法。通过标准加入法进行定量。通过用去活熔融石英衬里顶空进样器到气相色谱仪的传输管以及向样品瓶中添加甲酸，使拖尾现象最小化。用硫酸锂使样品饱和可提高回收率。该方法用于分析从早产儿收集的少量粪便。该技术的优点是快速、准确、可批量分析粪便标本，且样品制备最少。
