Imazeki N, Takeuchi A, Senoo A, Fuse Y
Second Department of Pathology, National Defense Medical College, Tokorozawa, Japan.
J Histochem Cytochem. 1994 Mar;42(3):329-35. doi: 10.1177/42.3.8308249.
The follicular dendritic cells (FDC) in B-lymphoid follicles are unique reticular cells that retain immune complexes on their surfaces. We developed new monoclonal antibodies (MAb), SKY01, 28, 41, and 49, against mouse FDC without useful cell markers. Immunohistochemical study of spleen and lymph node tissues from Balb/c, C3H, and C57BL/6 mice revealed that SKY01 and 49 were highly specific for FDC, whereas SKY28 and 41 reacted with other stromal components as well as FDC. On immunoelectron microscopy (IEM), reaction products for all MAb were localized on the surfaces of FDC. In ontogenetic study of Balb/c spleen, FDC precursors were not immunodetected with any of the MAb at 1 week after birth. The FDC that first appeared as immune complex-retaining cells at 2 weeks were a subpopulation of SKY01-, 49-, 28+, and 41+ reticular cells. At 3 weeks, FDC were positive for all MAb, like adult spleen. These results indicate that our MAb recognize differentiation antigens of FDC. Comparative immunohistochemical studies of spleen from athymic nude and severe combined immunodeficiency mice suggested that B-cells may be required for the differentiation of FDC. Consequently, these MAb are considered useful tools for research on FDC.
B淋巴细胞滤泡中的滤泡树突状细胞（FDC）是一种独特的网状细胞，其表面保留免疫复合物。我们开发了针对小鼠FDC的新型单克隆抗体（MAb）SKY01、28、41和49，这些FDC缺乏有用的细胞标志物。对来自Balb/c、C3H和C57BL/6小鼠的脾脏和淋巴结组织进行免疫组织化学研究发现，SKY01和49对FDC具有高度特异性，而SKY28和41不仅与FDC反应，还与其他基质成分反应。在免疫电子显微镜（IEM）下，所有MAb的反应产物都定位在FDC的表面。在Balb/c脾脏的个体发育研究中，出生后1周时，用任何一种MAb都无法免疫检测到FDC前体。在2周时首次作为免疫复合物保留细胞出现的FDC是SKY01-、49-、28+和41+网状细胞的一个亚群。在3周时，FDC对所有MAb均呈阳性，与成年脾脏相似。这些结果表明，我们的MAb识别FDC的分化抗原。对无胸腺裸鼠和严重联合免疫缺陷小鼠脾脏的比较免疫组织化学研究表明，FDC的分化可能需要B细胞。因此，这些MAb被认为是研究FDC的有用工具。
