Kapasi Z F, Burton G F, Shultz L D, Tew J G, Szakal A K
Department of Anatomy, Medical College of Virginia, Virginia Commonwealth University, Richmond.
J Immunol. 1993 Apr 1;150(7):2648-58.
Ag injected into immune mice immediately complexes with specific antibody. Immune complexes not phagocytosed by macrophages are transported by Ag transport cells to lymph node follicles for trapping by follicular dendritic cells (FDC). These FDC serve as a long term repository of unprocessed Ag that is believed to maintain both B cell memory and the secondary antibody response. Severe combined immunodeficiency mice lack functional B cells and T cells. Consequently, this mutation also appears to affect the ability to produce Ag-retaining FDC. to assess B and T cell requirements for FDC development and function, severe combined immunodeficiency mice were reconstituted with BM, or mature B and T cells. The development of a FDC reticulum, a three-dimensional network produced by the intertwining of FDC dendrites was assessed by Ag trapping on FDC using the histochemically detectable Ag horseradish peroxidase and quantitated by morphometry. The results showed that bone marrow transplants or B and T cells transferred together supply the required elements for the development of severe combined immunodeficiency FDC reticula that function in Ag trapping and the induction of the germinal center. In contrast, B cells or T cells injected separately induced a minimal development of FDC reticulum. The B and T cell requirements demonstrated here strongly indicate that B-T cell collaboration and the factors these cells produce are essential for FDC development.
注入免疫小鼠体内的抗原会立即与特异性抗体形成复合物。未被巨噬细胞吞噬的免疫复合物会被抗原转运细胞运输到淋巴结滤泡，由滤泡树突状细胞（FDC）捕获。这些FDC作为未处理抗原的长期储存库，据信可维持B细胞记忆和二次抗体反应。严重联合免疫缺陷小鼠缺乏功能性B细胞和T细胞。因此，这种突变似乎也会影响产生保留抗原的FDC的能力。为了评估FDC发育和功能对B细胞和T细胞的需求，用骨髓或成熟的B细胞和T细胞重建严重联合免疫缺陷小鼠。通过使用组织化学可检测的抗原辣根过氧化物酶在FDC上捕获抗原，并通过形态计量学进行定量，来评估由FDC树突交织产生的三维网络FDC网状结构的发育情况。结果表明，骨髓移植或一起转移的B细胞和T细胞为严重联合免疫缺陷FDC网状结构的发育提供了所需的元素，这些网状结构在抗原捕获和生发中心诱导中发挥作用。相比之下，单独注射B细胞或T细胞只会诱导FDC网状结构的最小发育。此处证明的B细胞和T细胞需求强烈表明，B - T细胞协作以及这些细胞产生的因子对于FDC发育至关重要。
