Takahashi K, Takenaka M, Takahashi K
Hiroshima Assisted Reproductive Technology Clinic.
Nihon Sanka Fujinka Gakkai Zasshi. 1994 Jan;46(1):21-6.
Two hundred forty-two cases of subzonal insemination (SUZI) were performed in 172 infertile male patients. Criteria for selection of subjects to receive SUZI were as follows; those who had previously failed to achieve fertilization by conventional in vitro fertilization (IVF) with normal semen analyses (group 1, n = 36), those who had previous fertilization failure in conventional IVF with abnormal semen analyses (group 2, n = 49) and those who had not been accepted for IVF because of severely abnormal semen analyses (group 3, n = 87). Two to twenty spermatozoa were injected into the perivitelline space of metaphase II oocytes. Fertilization occurred in 77.3% of patients and 40.9% of 1,220 eggs and 28 clinical pregnancies were established. There were no significant differences in fertilization rates, cleavage rates and embryo transfer rates among the three groups. However, the pregnancy rate was significantly lower in group 1 (7.7%) than in group 2 (20.0%) and group 3 (14.5%) (p < 0.05). These results indicated that SUZI improved the incidence of fertilization in male infertility. However, the fact that the lowest pregnancy rate was in group 1 indicates that further studies of patients with idiopathic failure of fertilization are needed.
对172例男性不育患者进行了242次卵周隙内单精子注射（SUZI）。接受SUZI治疗的受试者选择标准如下：既往精液分析正常但常规体外受精（IVF）未能受精者（第1组，n = 36）；既往精液分析异常且常规IVF受精失败者（第2组，n = 49）；因精液分析严重异常未被接受IVF者（第3组，n = 87）。将2至20条精子注射到中期II卵母细胞的卵周隙内。77.3%的患者发生受精，1220枚卵子中有40.9%受精，并确立了28例临床妊娠。三组之间的受精率、卵裂率和胚胎移植率无显著差异。然而，第1组的妊娠率（7.7%）显著低于第2组（20.0%）和第3组（14.5%）（p < 0.05）。这些结果表明，SUZI提高了男性不育患者的受精率。然而，第1组妊娠率最低这一事实表明，需要对特发性受精失败患者进行进一步研究。
