Riesbeck K, Gullberg M, Forsgren A
Department of Medical Microbiology, Lund University, Malmö General Hospital, Umeå, Sweden.
Transplantation. 1994 Jan;57(2):267-72. doi: 10.1097/00007890-199401001-00020.
The fluoroquinolone antibiotic ciprofloxacin (cipro) has been reported to upregulate interleukin 2 and interferon-gamma production in lectin-stimulated lymphocytes. The aim of this study was to elucidate whether cipro and the immunosuppressive agent CsA have antagonistic action on cytokine synthesis. Accumulation of IL-2 and IFN-gamma protein and mRNA were analyzed in polyclonally (PHA or Con A) or alloantigen-stimulated human peripheral blood lymphocytes. CsA added simultaneously with PHA partially blocked cytokine synthesis. The present study also shows that cipro supplemented with CsA and PHA resulted in significant higher concentrations of IL-2 (up to 60 times) and IFN-gamma (4.3 times) as compared with PHA and CsA alone. Similar results were obtained with primary mixed lymphocyte reactions. In parallel, a greater amount of IL-2 and IFN-gamma mRNA was observed in lymphocytes incubated with both cipro and CsA as compared with CsA alone. Our results reveal that CsA-dependent inhibition of both IL-2 and IFN-gamma expression is counteracted by high concentrations of cipro. These findings may be of importance in clinical transplantation.
据报道，氟喹诺酮类抗生素环丙沙星（环丙）可上调凝集素刺激的淋巴细胞中白细胞介素2和γ干扰素的产生。本研究的目的是阐明环丙沙星和免疫抑制剂环孢素A（CsA）对细胞因子合成是否具有拮抗作用。在多克隆（植物血凝素或刀豆蛋白A）或同种异体抗原刺激的人外周血淋巴细胞中分析白细胞介素2和γ干扰素蛋白及信使核糖核酸的积累情况。与植物血凝素同时添加的环孢素A部分阻断了细胞因子的合成。本研究还表明，与单独使用植物血凝素和环孢素A相比，添加环丙沙星、环孢素A和植物血凝素可使白细胞介素2（高达60倍）和γ干扰素（4.3倍）的浓度显著升高。在初次混合淋巴细胞反应中也得到了类似的结果。同时，与单独使用环孢素A相比，在同时用环丙沙星和环孢素A孵育的淋巴细胞中观察到了更多的白细胞介素2和γ干扰素信使核糖核酸。我们的结果显示，高浓度的环丙沙星可抵消环孢素A对白细胞介素2和γ干扰素表达的依赖性抑制作用。这些发现可能在临床移植中具有重要意义。
