Gruys E, Schakenraad J M, Kruit L K, Bolscher J M
Department of Veterinary Pathology, University of Utrecht, The Netherlands.
Vet Rec. 1993 Oct 16;133(16):385-8. doi: 10.1136/vr.133.16.385.
The biocompatibility of electronic transponders encapsulated in two different types of glass was studied after they had been implanted subcutaneously into pigs for the purpose of identification. Rods of white crystal glass or green iron-containing glass were screened for superficial impurities by scanning electron microscopy and X-ray analysis, revealing a few crystalline and plaque impurities which were similar for both types of glass, and no differences in elemental composition. In vitro cytotoxicity tests using cell cultures of human dermal fibroblasts and haemolysis and clot formation tests in human blood after contact with the rods, showed that both types of glass were biocompatible. When implanted subcutaneously at the base of the ears of pigs for from three to 150 days, both types of transponder appeared to induce a similar connective tissue capsule, on average less than 0.2 mm in thickness, surrounding the rods. A classic foreign body reaction did not occur. It is concluded that the fibrous capsules were due to scar tissue formed around the glass rods as a result of the tissue being damaged when they were implanted. There were no significant differences between the reactions to the two types of glass. The subcutaneous implantation of glass-encapsulated transponders appears to be a good method for identifying pigs.
为了进行识别，将封装在两种不同类型玻璃中的电子应答器皮下植入猪体内后，研究了其生物相容性。通过扫描电子显微镜和X射线分析对白色水晶玻璃或绿色含铁玻璃棒进行表面杂质筛查，发现两种类型的玻璃都有一些晶体和斑块杂质，且元素组成无差异。使用人皮肤成纤维细胞的细胞培养进行的体外细胞毒性试验以及与玻璃棒接触后人血中的溶血和凝血形成试验表明，两种类型的玻璃都具有生物相容性。当将两种应答器皮下植入猪耳根部3至150天时，两种应答器似乎都诱导形成了类似的结缔组织囊，平均厚度小于0.2毫米，包裹着玻璃棒。未发生典型的异物反应。得出的结论是，纤维囊是由于植入玻璃棒时组织受损，在其周围形成的瘢痕组织所致。对两种类型玻璃的反应之间没有显著差异。皮下植入玻璃封装的应答器似乎是一种识别猪的好方法。
