Tait I S, Evans G S, Flint N, Campbell F C
University Department of Surgery, Ninewells Hospital and Medical School, Dundee, Scotland.
Am J Surg. 1994 Jan;167(1):67-72. doi: 10.1016/0002-9610(94)90055-8.
A novel method of colonic mucosal replacement by transplantation of disaggregated small intestinal epithelium is described. Thirty-one inbred rats had the ascending colon isolated, and surgical mucosectomy was performed on the "free" loop. Epithelial cell aggregates were isolated from postnatal small intestine using collagenase and dispase digestion, then 20 microL of the cell suspension was "seeded" over the denuded colonic muscle of 25 recipient rats. Six control rats had surgical mucosectomy only. All loops were retrieved after 14 days for histologic examination. Stem cell lineage studies were used with selective staining protocols to identify enterocytes, goblet cells, entero-endocrine cells, and Paneth cells. A neomucosa with typical small bowel morphology including crypts and villi and all four stem cell lineages was regenerated by transplanted cells on the colonic muscle in 19 of 25 (76%) recipients. Control loops showed no epithelial regrowth confirming total mucosectomy. With appropriate stromal support, transplanted small intestinal stem cells have the capacity to re-epithelialize denuded colonic muscle with small bowel neomucosa.
本文描述了一种通过移植分散的小肠上皮进行结肠黏膜置换的新方法。31只近交系大鼠的升结肠被分离出来，对“游离”肠袢进行手术黏膜切除术。使用胶原酶和dispase消化法从出生后的小肠中分离上皮细胞聚集体，然后将20微升细胞悬液“接种”到25只受体大鼠裸露的结肠肌层上。6只对照大鼠仅进行手术黏膜切除术。14天后取出所有肠袢进行组织学检查。使用干细胞谱系研究和选择性染色方案来识别肠上皮细胞、杯状细胞、肠内分泌细胞和潘氏细胞。25只受体大鼠中有19只（76%）的结肠肌层上移植的细胞再生出了具有典型小肠形态（包括隐窝和绒毛）以及所有四种干细胞谱系的新黏膜。对照肠袢未显示上皮再生，证实了完全黏膜切除术。在适当的基质支持下，移植的小肠干细胞有能力用小肠新黏膜使裸露的结肠肌层重新上皮化。
