Mirza S H, Hannan A, Rizvi F
Armed Forces Institute of Pathology, Rawalpindi, Pakistan.
Ann Trop Med Parasitol. 1993 Oct;87(5):483-6. doi: 10.1080/00034983.1993.11812799.
Forty-two gastric biopsies, in two transport media, were homogenized and cultured on three media under micro-aerophilic conditions. Brain-heart infusion agar with a commercial antibiotic supplement (giving 10 mg vancomycin, 5 mg trimethoprim and 2500 i.u. polymixin per litre) yielded the best results. Ordinary chocolate (heated) human blood agar could be used in laboratories with limited resources. Growth was obtained in 4-6 days at 37 degrees C. All isolates were sensitive to metronidazole. The resistance to nalidixic acid and rapid urease production of Helicobacter pylori could be used to differentiate this species from Campylobacter spp. Indicator medium (brain-heart infusion broth with 7% human blood and 40 mg 2,3,5-triphenyletetrazolium chloride agar/litre) also proved useful in identification.
42份置于两种运输培养基中的胃活检样本被匀浆，并在微需氧条件下于三种培养基上进行培养。添加市售抗生素补充剂（每升含10mg万古霉素、5mg甲氧苄啶和2500国际单位多粘菌素）的脑心浸液琼脂产生了最佳结果。普通巧克力（加热）人血琼脂可用于资源有限的实验室。在37摄氏度下4 - 6天可获得生长。所有分离株对甲硝唑敏感。幽门螺杆菌对萘啶酸的耐药性和快速尿素酶产生可用于将该菌与弯曲杆菌属菌种区分开来。指示培养基（含7%人血和每升40mg氯化三苯四氮唑琼脂的脑心浸液肉汤）在鉴定中也被证明是有用的。
