生物样品在缓冲溶液中与固体支持物的共价结合用于扫描探针显微镜检查。

Karrasch S, Dolder M, Schabert F, Ramsden J, Engel A

Maurice E. Müller-Institute for High-Resolution Electron Microscopy, University of Basel, Switzerland.

Biophys J. 1993 Dec;65(6):2437-46. doi: 10.1016/S0006-3495(93)81327-4.

Scanning force microscopy allows imaging of biological molecules in their native state in buffer solution. To this end samples have to be fixed to a flat solid support so that they cannot be displaced by the scanning tip. Here we describe a method to achieve the covalent binding of biological samples to glass surfaces. Coverslips were chemically modified with the photoactivatable cross-linker N-5-azido-2-nitrobenzoyloxysuccinimide. Samples are squeezed between derivatized coverslips and then cross-linked to the glass surface by irradiation with ultraviolet light. Such samples can be imaged repeatedly by the scanning force microscope without loss of image quality, whereas identical but not immobilized samples are pushed away by the stylus.

扫描力显微镜能够对缓冲溶液中处于天然状态的生物分子进行成像。为此，样品必须固定在平坦的固体支持物上，以使它们不会被扫描探针移位。在此，我们描述一种实现生物样品与玻璃表面共价结合的方法。用可光活化交联剂N-5-叠氮基-2-硝基苯甲酰氧基琥珀酰亚胺对盖玻片进行化学修饰。将样品挤压在衍生化的盖玻片之间，然后通过紫外线照射交联到玻璃表面。这样的样品可以用扫描力显微镜反复成像而不会损失图像质量，而相同但未固定的样品会被探针推开。