Ohya T
Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Biol Pharm Bull. 1993 Nov;16(11):1078-82. doi: 10.1248/bpb.16.1078.
The reactivity of formaldehyde (FA), acetaldehyde (AA) and propanal (PA) towards malondialdehyde (MDA) under physiological conditions was investigated. These alkanals revealed a reactivity towards MDA. Especially, FA showed high reactivity; for instance, a mixture of MDA (1 mM) and FA (6 mM) incubated for 4h, or a mixture of MDA (1 mM) and FA (1 mM) incubated for 24 h, resulted in almost a 100% decrease of MDA. The amount of free MDA decreased as the alkanal concentration increased and in the reaction of MDA with AA or PA, the loss of MDA was in approximate agreement with the yield of 2,4-dihydroxymethylene-3-alkylglutaraldehyde (1b or 1c). In the reaction of MDA with FA, the yield of 1a decreased with an excess amount of FA. In a thiobarbituric acid (TBA) test, 1 gave a positive reaction. Compounds 1b and 1c, which contain 2 molecules of MDA, liberated 1 molecule of MDA during the TBA reaction. The results showed that the MDA recovery of 1b and 1c with the TBA test was 50%. In the case of 1a, MDA recovery was 32%. In the MDA/p-nitrophenylhydrazine (NPH)-HPLC method, the MDA recovery of 1a, 1b and 1c ranged from 3-7%.
研究了甲醛（FA）、乙醛（AA）和丙醛（PA）在生理条件下对丙二醛（MDA）的反应活性。这些链烷醛对MDA表现出反应活性。特别是，FA显示出高反应活性；例如，将MDA（1 mM）和FA（6 mM）的混合物孵育4小时，或将MDA（1 mM）和FA（1 mM）的混合物孵育24小时，导致MDA几乎100%减少。随着链烷醛浓度的增加，游离MDA的量减少，并且在MDA与AA或PA的反应中，MDA的损失与2,4-二羟基亚甲基-3-烷基戊二醛（1b或1c）的产率大致一致。在MDA与FA的反应中，1a的产率随着过量FA而降低。在硫代巴比妥酸（TBA）试验中，1产生阳性反应。含有2分子MDA的化合物1b和1c在TBA反应过程中释放出1分子MDA。结果表明，用TBA试验对1b和1c的MDA回收率为50%。在1a的情况下，MDA回收率为32%。在MDA/对硝基苯肼（NPH）-HPLC方法中，1a、1b和1c的MDA回收率为3-7%。
