Ationu A, Sorensen K, Whitehead B, Singer D, Burch M, Carter N D
Child Health Department, St George's Hospital, London, United Kingdom.
Cardiovasc Res. 1993 Dec;27(12):2135-9. doi: 10.1093/cvr/27.12.2135.
The aim was to examine ventricular brain natriuretic peptide (B-type natriuretic peptide, BNP) gene expression and to determine its relationship with ventricular BNP and circulating BNP levels in paediatric cardiac transplant recipients, over a three year period after transplantation.
Total RNA extracted from endomyocardial right ventricular biopsy tissues (n = 26) of 13 cardiac transplant recipients (age range 5-17 years) and 10 normal hearts obtained at necropsy (age range 19-76 years) as controls was analysed by northern and slot blot hybridisations. Specific radioimmunoassay techniques were used to determine levels of BNP and atrial natriuretic peptide (A-type natriuretic peptide, ANP) in plasma (n = 26) and ventricular biopsy (n = 26) samples.
Ventricular BNP messenger ribonucleic acid (mRNA) levels from slot blot hybridisations in the transplanted heart [122(3) arbitrary units, range 97-143] were significantly higher (p < 0.01) than in the normal heart [63(5) arbitrary units, range 37-98]. Northern blot hybridisations confirmed this result and gave a major BNP mRNA transcript of approximately 900 nucleotides. There was no significant relationship between ventricular BNP mRNA levels and ventricular BNP (r = 0.15, p = 0.5, n = 26) or plasma BNP levels (r = 0.16, p = 0.4, n = 26). There was also no significant relationship between ventricular BNP mRNA levels and any of the haemodynamic variables, or immunosuppressive drugs. A ventricular ANP RNA transcript of approximately 900 nucleotides was detected in the transplanted heart but was below the limit of detection in the normal heart. For the long term study, increased levels of BNP and ANP in both plasma and ventricular samples were observed in the first year after transplantation, with a significant reduction (p < 0.01) in levels three years later.
Ventricular BNP gene expression is increased at the mRNA level after heart transplantation in children. Expression of both ventricular BNP mRNA and ANP mRNA in the transplanted heart may be an important response in the modulation of cardiac function after transplantation.
旨在研究小儿心脏移植受者移植后三年内心室脑钠肽（B型钠尿肽，BNP）基因表达情况，并确定其与心室BNP及循环BNP水平的关系。
采用Northern杂交和狭缝杂交分析法，对13例心脏移植受者（年龄范围5 - 17岁）右心室心内膜活检组织（n = 26）以及10例尸检获得的正常心脏（年龄范围19 - 76岁）提取的总RNA进行分析。采用特异性放射免疫测定技术测定血浆（n = 26）和心室活检样本（n = 26）中BNP和心房钠尿肽（A型钠尿肽，ANP）水平。
狭缝杂交法检测移植心脏心室BNP信使核糖核酸（mRNA）水平为122（3）任意单位，范围97 - 143，显著高于正常心脏[63（5）任意单位，范围37 - 98]（p < 0.01）。Northern杂交证实了这一结果，并得到一条约900个核苷酸的主要BNP mRNA转录本。心室BNP mRNA水平与心室BNP（r = 0.15，p = 0.5，n = 26）或血浆BNP水平（r = 0.16，p = 0.4，n = 26）之间无显著相关性。心室BNP mRNA水平与任何血流动力学变量或免疫抑制药物之间也无显著相关性。在移植心脏中检测到一条约900个核苷酸的心室ANP RNA转录本，但在正常心脏中低于检测限。在长期研究中，移植后第一年血浆和心室样本中BNP和ANP水平均升高，三年后水平显著降低（p < 0.01）。
儿童心脏移植后心室BNP基因在mRNA水平表达增加。移植心脏中心室BNP mRNA和ANP mRNA的表达可能是移植后心脏功能调节的重要反应。
