Nakon R, Krishnamoorthy C R
Science. 1983 Aug 19;221(4612):749-50. doi: 10.1126/science.6879173.
Metal-ion affinity (formation) constants were determined for two "Good's" buffers, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) and N,N-bis(2-hydroxyethyl)glycine (bicine). The metal chelates formed undergo loss of an internal ligand (alcohol) proton (bicine) and undergo hydrolysis (bicine and TES) and dimerization reactions (TES). Bicine and TES buffer not only hydrogen ions but also metal ions. The metal complexes of "Good's" buffers also buffer hydrogen ions by secondary reactions. The consequences of these reactions are considered in relation to biomedical research.
测定了两种“Good's”缓冲剂，即N-三（羟甲基）甲基-2-氨基乙磺酸（TES）和N，N-双（2-羟乙基）甘氨酸（二乙醇胺）的金属离子亲和（形成）常数。形成的金属螯合物会失去一个内部配体（醇）质子（二乙醇胺），并发生水解（二乙醇胺和TES）和二聚反应（TES）。二乙醇胺和TES缓冲剂不仅能缓冲氢离子，还能缓冲金属离子。“Good's”缓冲剂的金属络合物也通过二级反应缓冲氢离子。这些反应的后果与生物医学研究相关。
