Dinjens W N, van der Weiden M M, Schroeder F H, Bosman F T, Trapman J
Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
Int J Cancer. 1994 Mar 1;56(5):630-3. doi: 10.1002/ijc.2910560504.
We have studied the frequency of mutations in the p53 gene in human prostate cancer. The investigated material consisted of 20 primary-tumor tissue specimens, obtained by transurethral resection and tissue specimens of 15 lymph-node metastases, obtained at total prostatectomy. The applied methods encompassed immunohistochemistry on frozen sections, using the monoclonal antibody PAb 1801, and single-strand conformation polymorphism (SSCP) analysis, after amplification of single exon sequences by PCR, on exons 5 to 8 of the p53 gene. The mutations, leading to aberrantly migrating bands in the PCR-SSCP analysis, were identified by direct sequencing of the PCR product. Immunohistochemical and PCR-SSCP analysis were completely confirmative. In the primary tumors, mutations were found in 10% of the specimens (codons 232 and 273), and in lymph-node metastases in 15% of the specimens (codons 248 and 273). In one case (codon 273), the same mutation was found both in the primary tumor and in the lymph-node metastasis. Our results show that p53 mutations are infrequent in both primary and metastatic prostate tumors. In addition, they indicate that there is no strict correlation between p53 mutation and tumor metastasis.
我们研究了人类前列腺癌中p53基因的突变频率。所研究的材料包括20份经尿道切除获得的原发性肿瘤组织标本，以及15份在前列腺全切术中获得的淋巴结转移灶组织标本。应用的方法包括在冰冻切片上进行免疫组织化学分析，使用单克隆抗体PAb 1801，以及在通过PCR扩增p53基因第5至8外显子的单外显子序列后，进行单链构象多态性（SSCP）分析。通过对PCR产物进行直接测序来鉴定在PCR-SSCP分析中导致异常迁移条带的突变。免疫组织化学分析和PCR-SSCP分析结果完全一致。在原发性肿瘤中，10%的标本（密码子232和273）发现有突变，在淋巴结转移灶中，15%的标本（密码子248和273）发现有突变。在1例（密码子273）中，在原发性肿瘤和淋巴结转移灶中均发现相同的突变。我们的结果表明，p53突变在原发性和转移性前列腺肿瘤中均不常见。此外，结果表明p53突变与肿瘤转移之间没有严格的相关性。
