Porta G, Zucchi I, Hillier L, Green P, Nowotny V, D'Urso M, Schlessinger D
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Genomics. 1993 May;16(2):417-25. doi: 10.1006/geno.1993.1205.
The contents of Alu- and L1-containing TaqI restriction fragments were assessed by Southern blot analyses across YAC contigs already assembled by other means and localized within Xq24-q28. Fingerprinting patterns of YACs in contigs were concordant, and using software based on that of M. V. Olson et al. (1986, Proc. Natl. Acad. Sci. USA 83: 7826) to analyze digitized data on fragment sizes, fingerprinting itself could establish matches among about 40% of a test group of 435 YACs. At 100-kb resolution, both repetitive elements were found throughout the region, with no apparent enrichment of Alu or L1 in DNA of G compared to that found in R bands. However, consistent with a random overall distribution, delimited regions of up to 100 kb contained clusters of repetitive elements. The local concentrations may help to account for the reported differential hybridization of Alu and L1 probes to segments of metaphase chromosomes.
通过Southern印迹分析，对已通过其他方法组装并定位在Xq24 - q28内的YAC重叠群中含Alu和L1的TaqI限制性片段的含量进行了评估。重叠群中YAC的指纹图谱是一致的，并且使用基于M. V. Olson等人（1986年，《美国国家科学院院刊》83: 7826）的软件来分析片段大小的数字化数据，指纹图谱本身可以在435个YAC的测试组中约40%的YAC之间建立匹配。在100 kb的分辨率下，在整个区域都发现了这两种重复元件，与R带中的DNA相比，G带DNA中Alu或L1没有明显富集。然而，与随机的总体分布一致，长达100 kb的划定区域包含重复元件簇。局部浓度可能有助于解释所报道的Alu和L1探针与中期染色体片段的差异杂交。
