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对人和动物葡萄球菌进行检测时金黄色葡萄球菌的快速鉴定方法:与一种新的免疫酶测定法的比较

Rapid methods for identification of Staphylococcus aureus when both human and animal staphylococci are tested: comparison with a new immunoenzymatic assay.

作者信息

Guardati M C, Guzmàn C A, Piatti G, Pruzzo C

机构信息

Institute of Microbiology, University of Genova, Italy.

出版信息

J Clin Microbiol. 1993 Jun;31(6):1606-8. doi: 10.1128/jcm.31.6.1606-1608.1993.

DOI:10.1128/jcm.31.6.1606-1608.1993
PMID:8315003
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265586/
Abstract

A new immunoenzymatic assay (IEA) for the identification of Staphylococcus aureus strains of both human and animal origin was compared with rapid commercial kits. The sensitivities and specificities of the commercial kits varied from 90.2 to 96% and 90.8 to 93.7%, respectively. The IEA did not give any false-negative or false-positive results, while commercial kits gave high percentages of false-positive results among clumping factor-positive non-S. aureus strains. The IEA is particularly useful for isolates for which identification is doubtful, for large-scale epidemiological studies, and for identifying isolates from animals as S. aureus.

摘要

一种用于鉴定人和动物源金黄色葡萄球菌菌株的新型免疫酶测定法(IEA)与快速商用试剂盒进行了比较。商用试剂盒的灵敏度和特异性分别在90.2%至96%和90.8%至93.7%之间。IEA未产生任何假阴性或假阳性结果,而商用试剂盒在凝聚因子阳性的非金黄色葡萄球菌菌株中产生了较高比例的假阳性结果。IEA对于鉴定存疑的分离株、大规模流行病学研究以及将动物分离株鉴定为金黄色葡萄球菌特别有用。

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本文引用的文献

1
Occurrence of protein a in staphylococcal strains: quantitative aspects and correlation to antigenic and bacteriophage types.葡萄球菌株中蛋白 A 的出现:定量方面及其与抗原和噬菌体型的相关性。
Infect Immun. 1971 Jan;3(1):10-5. doi: 10.1128/iai.3.1.10-15.1971.
2
Significance of protein a production by staphylococci.葡萄球菌产生蛋白 A 的意义。
Infect Immun. 1970 Nov;2(5):672-3. doi: 10.1128/iai.2.5.672-673.1970.
3
Evaluation of a commercial latex agglutination test for identification of Staphylococcus aureus.一种用于鉴定金黄色葡萄球菌的商用乳胶凝集试验的评估。
J Clin Microbiol. 1982 Mar;15(3):416-8. doi: 10.1128/jcm.15.3.416-418.1982.
4
Purification and characterization of three separate bacteriolytic enzymes excreted by Staphylococcus aureus, Staphylococcus simulans, and Staphylococcus saprophyticus.金黄色葡萄球菌、模仿葡萄球菌和腐生葡萄球菌分泌的三种不同溶菌酶的纯化与特性分析
J Bacteriol. 1982 Aug;151(2):636-47. doi: 10.1128/jb.151.2.636-647.1982.
5
Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains.致敏绵羊红细胞作为金黄色葡萄球菌蛋白A的反应物。方法学与流行病学,特别提及弱反应性耐甲氧西林菌株
Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):150-6.
6
Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的各种快速凝集方法的评估。
J Clin Microbiol. 1985 May;21(5):726-9. doi: 10.1128/jcm.21.5.726-729.1985.
7
Comparison of a yellow latex reagent with other agglutination methods for the identification of Staphylococcus aureus.一种黄色乳胶试剂与其他凝集方法用于鉴定金黄色葡萄球菌的比较。
J Clin Microbiol. 1986 Mar;23(3):640-2. doi: 10.1128/jcm.23.3.640-642.1986.
8
Performance of four slide agglutination methods for identification of Staphylococcus aureus when testing methicillin-resistant staphylococci.检测耐甲氧西林葡萄球菌时,四种玻片凝集法用于鉴定金黄色葡萄球菌的性能。
J Clin Microbiol. 1987 Jan;25(1):181-2. doi: 10.1128/jcm.25.1.181-182.1987.
9
Evaluation of rapid coagulase methods for the identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的快速凝固酶方法的评估。
J Clin Microbiol. 1986 May;23(5):916-9. doi: 10.1128/jcm.23.5.916-919.1986.
10
Problems with rapid agglutination methods for identification of Staphylococcus aureus when Staphylococcus saprophyticus is being tested.在检测腐生葡萄球菌时,用于鉴定金黄色葡萄球菌的快速凝集方法存在的问题。
J Clin Microbiol. 1988 Jul;26(7):1398-9. doi: 10.1128/jcm.26.7.1398-1399.1988.