Akabane H, Nagao M, Zhang W D, Yamada Y, Oono T, Takatori T
Obihiro Kyokai Hospital, Japan.
Nihon Hoigaku Zasshi. 1993 Apr;47(2):93-7.
A sensitive radioimmunoassay was developed for the determination of leukotoxin (9: 10-epoxy-12-octadecenoic acid), which was reported to exist in human burned skin and neutrophils, and was regarded as a toxic and/or defensive substance in living beings. The leukotoxin was conjugated with bovine serum albumin by means of the mixed anhydride technique as immunogen and rabbits were immunized over 4 months. According to a titration test of antiserum, forty-fold diluted antiserum was found to bind approximately 50% of about 4,000 dpm of methylated leukotoxin labeled with [14C] carbon. The detection limit of leukotoxin was at least as low as 5 ng in this radioimmunoassay by use of a polyetylene glycol precipitation method. This antiserum had a strong specificity to leukotoxin and no cross-reactivity with the other analogs tested.
已开发出一种灵敏的放射免疫分析法，用于测定白细胞毒素（9:10-环氧-12-十八碳烯酸）。据报道，该毒素存在于人类烧伤皮肤和中性粒细胞中，被视为生物体内的一种有毒和/或防御性物质。通过混合酸酐技术将白细胞毒素与牛血清白蛋白偶联作为免疫原，并对兔子进行了4个月的免疫。根据抗血清的滴定试验，发现40倍稀释的抗血清能结合约4000 dpm[14C]碳标记的甲基化白细胞毒素的约50%。在该放射免疫分析法中，采用聚乙二醇沉淀法时，白细胞毒素的检测限至少低至5 ng。这种抗血清对白细胞毒素具有很强的特异性，与所测试的其他类似物无交叉反应。
