[N-乙酰葡糖胺基-（β1-4）-N-乙酰胞壁酰-丙氨酰-D-异谷氨酰胺单克隆抗体的制备及特性]
[Preparation and characteristics of monoclonal antibodies to N-acetylglucosaminyl-(beta1-4)-N-acetylmuramoyl-alanyl-D-isoglutamine].
作者信息
Mareeva T Iu, Kotova O V, Makarov E A, Andronova T M, Nesmeianov V A
出版信息
Bioorg Khim. 1993 May;19(5):555-61.
PMID:8318024
Abstract
Hybridoma E6/1.2 was produced by fusion of splenocytes from mice immunized with N-acetylglucosaminyl-(beta 1-4)-N-acetylmuramyl-alanyl-D-isoglutamine (GMDP), conjugated to methylated BSA, with SP2/0 myeloma cells. GMDP-specific monoclonal antibody was IgG1 subtype and had affinity constant 2.10(9) M-1. According to competitive ELISA, the presence of the intact disaccharide fragment and the alanyl residue was critical for the GMDP-antibody interaction.
摘要
杂交瘤E6/1.2是通过将用与甲基化牛血清白蛋白（BSA）偶联的N-乙酰葡糖胺基-（β1-4）-N-乙酰胞壁酰-丙氨酰-D-异谷氨酰胺（GMDP）免疫的小鼠脾细胞与SP2/0骨髓瘤细胞融合而产生的。GMDP特异性单克隆抗体为IgG1亚型，亲和常数为2.1×10⁹ M⁻¹。根据竞争性酶联免疫吸附测定，完整二糖片段和丙氨酰残基的存在对GMDP与抗体的相互作用至关重要。
Zotero 插件