Nilsson O S, Dallner G
Biochim Biophys Acta. 1977 Jan 21;464(2):453-8. doi: 10.1016/0005-2736(77)90019-0.
Subcellular membranes isolated from rat liver in a form impermeable to macromolecules were treated with phospholipase A2 from Naja naja venom. The phosphatidylserine, phosphatidylethanolamine and about half of the phosphatidylcholine of microsomes, Golgi membranes, inner mitochondrial membranes, lysosomes and nuclear membranes were hydrolyzed. It is proposed that these phospholipids are localized in the outer surface of the membrane bilayer, which represents the cytoplasmic side in the living cell, while the remaining phosphatidylcholine and most of the phosphatidylinositol, sphingomyelin and cardiolipin may be assigned to the inner side of the bilayer.
从大鼠肝脏中分离出的对大分子不可渗透的亚细胞膜，用眼镜蛇毒中的磷脂酶A2进行处理。微粒体、高尔基体膜、线粒体内膜、溶酶体和核膜中的磷脂酰丝氨酸、磷脂酰乙醇胺以及大约一半的磷脂酰胆碱被水解。有人提出，这些磷脂位于膜双层的外表面，在活细胞中代表细胞质一侧，而其余的磷脂酰胆碱以及大部分的磷脂酰肌醇、鞘磷脂和心磷脂可能位于双层的内侧。
