Decker C, Miro Obradors M J, Sillence D J, Allan D
Department of Physiology, University College London, U.K.
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):885-90. doi: 10.1042/bj3200885.
The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated with phorbol 12-myristate 13-acetate and n-butanol. Results derived by isolation of plasma membrane vesicles from intact cells or by subcellular fractionation on a sucrose density gradient suggest the PLD is specific for phosphatidylcholine and its primary site of action is not the plasma membrane but the endoplasmic reticulum.
通过测定用佛波醇12 -肉豆蔻酸酯13 -乙酸酯和正丁醇孵育细胞时产生的磷脂丁醇的亚细胞分布，研究了佛波醇酯敏感的磷脂酶D（PLD）在幼仓鼠肾细胞中的定位。从完整细胞中分离质膜囊泡或通过蔗糖密度梯度进行亚细胞分级分离得到的结果表明，PLD对磷脂酰胆碱具有特异性，其主要作用位点不是质膜而是内质网。
