Gaspard P, Schwartzbrod J
Département de Microbiologie, WHO Collaborating Centre, Faculté de Pharmacie, Nancy, France.
Zentralbl Hyg Umweltmed. 1993 Mar;193(6):513-20.
In third world countries, the agricultural use of treated wastewater represents an interesting solution. A recent report of W.H.O. shows the importance of strict parasitological criteria for such reuse. The aim of this paper is to study the conditions under which Ascaris eggs have been recovered from artificially contaminated soils (sandy, clay or loamy soil, mould garden). The eggs elution has been carried out from soil particles using various solutions (detergents, distilled water, formaldehyde, sodium hydroxide, sodium hypochlorite). The recovery percentage analysis showed the superiority of the sodium hypochlorite solution titrating 10 chlorometric degrees, whatever the soil type. The eggs concentration in the eluates has been carried out through the flotation technique by testing various reagents with densities ranging from 1.16 to 1.44. The zinc sulphate solution at 50%, 55% and 66% prove to be flotation agents that are well adapted to this sampling type. By carrying out the elution with a sodium hypochlorite solution titrating 10 chlorometric degrees and the concentration by flotation with a zinc sulphate solution at 55% the recovery percentages, which are independent of the parasitic load, vary from 66 to 78%.
在第三世界国家，经处理的废水用于农业是一个有意思的解决方案。世界卫生组织最近的一份报告表明了严格的寄生虫学标准对于此类再利用的重要性。本文的目的是研究从人工污染土壤（沙土、黏土或壤土、花园土）中回收蛔虫卵的条件。已使用各种溶液（洗涤剂、蒸馏水、甲醛、氢氧化钠、次氯酸钠）从土壤颗粒中进行虫卵洗脱。回收率分析表明，无论土壤类型如何，滴定度为10度氯的次氯酸钠溶液具有优越性。通过测试密度范围为1.16至1.44的各种试剂，利用浮选技术对洗脱液中的虫卵进行浓缩。50%、55%和66%的硫酸锌溶液被证明是适用于这种采样类型的浮选剂。通过用滴定度为10度氯的次氯酸钠溶液进行洗脱，并使用55%的硫酸锌溶液进行浮选浓缩，与寄生虫负荷无关的回收率在66%至78%之间。
