牛α干扰素受体的克隆与特性分析
Cloning and characterization of a bovine alpha interferon receptor.
作者信息
Lim J K, Langer J A
机构信息
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854.
出版信息
Biochim Biophys Acta. 1993 Jun 25;1173(3):314-9. doi: 10.1016/0167-4781(93)90129-2.
A bovine interferon alpha receptor (BoIFN-alpha R1) cDNA, homologous to the human cDNA, was isolated. Transfection of the BoIFN-alpha R1 cDNA into monkey COS cells results in a large increase in high-affinity binding sites for human IFN-alpha A and IFN-alpha B. Covalent crosslinking of radiolabeled HuIFN-alpha A and -alpha B demonstrates that the complex of [32P]HuIFN with the BoIFN-alpha R1 protein (predicted mass, 61,375) expressed in COS cells migrates as a 140-150 kDa band.
分离出了一种与人类cDNA同源的牛干扰素α受体（BoIFN-αR1）cDNA。将BoIFN-αR1 cDNA转染到猴COS细胞中会导致人干扰素αA和干扰素αB的高亲和力结合位点大幅增加。放射性标记的HuIFN-αA和-αB的共价交联表明，在COS细胞中表达的[32P]HuIFN与BoIFN-αR1蛋白（预测质量为61375）的复合物迁移为140 - 150 kDa的条带。
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