Takahashi H, Aizawa S, Konishi E, Furusato M, Kato H, Ashihara T
Department of Pathology, Jikei University School of Medicine, Tokyo, Japan.
Cancer. 1993 Jul 15;72(2):549-52. doi: 10.1002/1097-0142(19930715)72:2<549::aid-cncr2820720235>3.0.co;2-l.
Whether spermatocytic seminoma (SS) develops during meiosis or not is still controversial. To determine that, the DNA content of large, small, and intermediate cells of SS was measured by cytofluorometry to obtain DNA histograms for each cell type. The large cells were aneuploid and showed cell cycles with G0/G1 DNA values from 3C to 18C. The ploidy of the large cells doubled stepwise (i.e., 4.5C-9C-18C, 3C-6C-12C, and so forth). The small cells showed distinct cell cycles with a DNA content of 2C or near 2C, and the intermediate cells showed both diploid and aneuploid DNA values. It is speculated that none of the cell types is involved in meiosis, based on the fact that each size of cell appears to have its own cell division cycle. It is concluded that in SS, the diploid cell cycle formation by small cells and the creation of large cells by continuous sequential polyploidization result in the morphologic expression of three types of cells (large, small, intermediate).
精母细胞性精原细胞瘤（SS）是否在减数分裂期间发生仍存在争议。为了确定这一点，通过细胞荧光测定法测量了SS的大、小和中等大小细胞的DNA含量，以获得每种细胞类型的DNA直方图。大细胞为非整倍体，显示出G0/G1期DNA值为3C至18C的细胞周期。大细胞的倍性逐步加倍（即4.5C - 9C - 18C、3C - 6C - 12C等）。小细胞显示出明显的细胞周期，DNA含量为2C或接近2C，中等大小细胞则显示出二倍体和非整倍体DNA值。基于每种大小的细胞似乎都有其自身的细胞分裂周期这一事实，推测没有任何一种细胞类型参与减数分裂。得出的结论是，在SS中，小细胞形成二倍体细胞周期以及通过连续的顺序多倍体化产生大细胞，导致了三种类型细胞（大、小、中等）的形态学表现。
