Dekker I, Rozeboom T, Delemarre J, Dam A, Oosterhuis J W
Department of Pathology, University of Groningen, The Netherlands.
Cancer. 1992 Feb 15;69(4):993-6. doi: 10.1002/1097-0142(19920215)69:4<993::aid-cncr2820690427>3.0.co;2-8.
Immunohistochemical analysis was done on 7 testicular tumors classified as spermatocytic seminoma (SS) and 25 classic seminomas. Except for a few scattered cells, the spermatocytic seminomas were negative for placental-like alkaline phosphatase (PLAP); the classic seminomas were all positive for this enzyme. The SS also were negative for alpha-fetoprotein (AFP), beta-human chorionic gonadotropin (hCG), and leukocyte common antigen (LCA). The ploidy of the seven tumors of SS was as follows: two, diploid; two, near-diploid; one, tetraploid; one, aneuploid; and one, uninterpretable. The essentially negative staining of SS for PLAP was strikingly different from the pattern in classic seminoma. Thus, staining for this enzyme is useful for making the differential diagnosis between classic seminoma and SS. To differentiate between malignant lymphoma and SS, staining for leukocyte common antigen is helpful.
对7例分类为精母细胞性精原细胞瘤（SS）和25例经典型精原细胞瘤的睾丸肿瘤进行了免疫组织化学分析。除少数散在细胞外，精母细胞性精原细胞瘤对胎盘样碱性磷酸酶（PLAP）呈阴性；经典型精原细胞瘤对此酶均呈阳性。SS对甲胎蛋白（AFP）、β-人绒毛膜促性腺激素（hCG）和白细胞共同抗原（LCA）也呈阴性。7例SS肿瘤的倍体情况如下：2例为二倍体；2例为近二倍体；1例为四倍体；1例为非整倍体；1例无法判断。SS对PLAP基本呈阴性染色与经典型精原细胞瘤的模式明显不同。因此，该酶的染色有助于经典型精原细胞瘤与SS之间的鉴别诊断。为了区分恶性淋巴瘤与SS，白细胞共同抗原染色是有帮助的。
