Wright R M, Repine T, Repine J E
Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver 80262.
Curr Genet. 1993 May-Jun;23(5-6):388-91. doi: 10.1007/BF00312623.
Pseudohyphal growth in Saccharomyces cerevisiae has been postulated to be an adaptation to foraging for nitrogen during nitrogen starvation. This process was described as a strictly diploid phenomenon which did not occur in haploid yeast cells and was under the genetic control of both the mating-type locus and a group of five genes, the BUD genes, regulating bud formation. We have also observed a dimorphic growth pattern in yeast growing on various nitrogen-limiting synthetic media. However, and in contrast to a previous report, we find that pseudohyphal growth is not precluded in haploid cells. We demonstrate that haploid pseudohyphal growth is strictly oxygen-dependent and is rapidly reversible, defining pseudohyphal growth as a reversible developmental pathway in yeast.
酿酒酵母中的假菌丝生长被认为是在氮饥饿期间对氮源搜寻的一种适应性反应。这个过程被描述为一种严格的二倍体现象，在单倍体酵母细胞中不会发生，并且受交配型基因座和一组五个调节芽形成的基因（即BUD基因）的遗传控制。我们也观察到酵母在各种氮限制合成培养基上生长时呈现出一种二态生长模式。然而，与之前的一份报告相反，我们发现单倍体细胞中并不排除假菌丝生长。我们证明单倍体假菌丝生长严格依赖氧气且可快速逆转，将假菌丝生长定义为酵母中一种可逆的发育途径。
