使用血型糖蛋白A人类突变试验研究接触苯乙烯的工人。
Use of the glycophorin A human mutation assay to study workers exposed to styrene.
作者信息
Compton-Quintana P J, Jensen R H, Bigbee W L, Grant S G, Langlois R G, Smith M T, Rappaport S M
机构信息
Department of Environmental Health Sciences, University of California, Berkeley 94720.
出版信息
Environ Health Perspect. 1993 Mar;99:297-301. doi: 10.1289/ehp.9399297.
The glycophorin A (GPA) assay is a human mutation assay that is potentially useful for large epidemiological studies. The assay is rapid and requires a minimal amount of blood, which can be stored before analysis. The data presented here were collected from workers exposed to styrene in a boat manufacturing plant. This study was the first to apply the GPA assay to an occupationally exposed population. Subjects with a mean styrene exposure of 30 ppm had a higher frequency of GPA N phi variant cells than subjects with mean exposure of 1 ppm, but the subjects differed in respect to smoking and age distribution. Results indicate that the original 1-W-1 version of the assay may not be suitable for studies of small numbers of exposed subjects due to variability and artifacts. The newer BR6 version, however, has much lower variability and shows promise for use in the occupational setting.
血型糖蛋白A（GPA）检测是一种人体突变检测方法，可能对大型流行病学研究有用。该检测方法快速，所需血量极少，且血液在分析前可保存。此处呈现的数据收集自一家船舶制造工厂中接触苯乙烯的工人。本研究首次将GPA检测应用于职业暴露人群。平均苯乙烯暴露量为30 ppm的受试者中GPA N phi变异细胞的频率高于平均暴露量为1 ppm的受试者，但两组受试者在吸烟和年龄分布方面存在差异。结果表明，由于存在变异性和假象，最初的1-W-1版本检测可能不适用于少量暴露受试者的研究。然而，更新的BR6版本变异性低得多，有望用于职业环境。
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