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结核分枝杆菌对异烟肼敏感性所需的过氧化氢酶-过氧化物酶编码基因katG的特征分析。

Characterization of the katG gene encoding a catalase-peroxidase required for the isoniazid susceptibility of Mycobacterium tuberculosis.

作者信息

Heym B, Zhang Y, Poulet S, Young D, Cole S T

机构信息

Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.

出版信息

J Bacteriol. 1993 Jul;175(13):4255-9. doi: 10.1128/jb.175.13.4255-4259.1993.

DOI:10.1128/jb.175.13.4255-4259.1993
PMID:8320241
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204858/
Abstract

The isoniazid susceptibility of Mycobacterium tuberculosis is mediated by the product of the katG gene which encodes the heme-containing enzyme catalase-peroxidase. In this study, the chromosomal location of katG has been established and its nucleotide sequence has been determined so that the primary structure of catalase-peroxidase could be predicted. The M. tuberculosis enzyme is an 80,000-dalton protein containing several motifs characteristic of peroxidases and shows strong similarity to other bacterial catalase-peroxidases. Expression of the katG gene in M. tuberculosis, M. smegmatis, and Escherichia coli was demonstrated by Western blotting (immunoblotting). Homologous genes were detected in other mycobacteria, even those which are naturally insensitive to isoniazid.

摘要

结核分枝杆菌对异烟肼的敏感性由katG基因的产物介导，该基因编码含血红素的酶过氧化氢酶-过氧化物酶。在本研究中，已确定katG的染色体定位并测定了其核苷酸序列，从而可以预测过氧化氢酶-过氧化物酶的一级结构。结核分枝杆菌的这种酶是一种80000道尔顿的蛋白质，含有几个过氧化物酶特有的基序，并且与其他细菌过氧化氢酶-过氧化物酶有很强的相似性。通过蛋白质印迹法（免疫印迹法）证实了katG基因在结核分枝杆菌、耻垢分枝杆菌和大肠杆菌中的表达。在其他分枝杆菌中也检测到了同源基因，甚至包括那些对异烟肼天然不敏感的分枝杆菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b277/204858/85a02268c13a/jbacter00055-0351-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b277/204858/6cf5bd07ef71/jbacter00055-0350-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b277/204858/85a02268c13a/jbacter00055-0351-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b277/204858/6cf5bd07ef71/jbacter00055-0350-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b277/204858/85a02268c13a/jbacter00055-0351-a.jpg

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