Baird K S, Crossan J F, Ralston S H
University Department of Orthopaedic Surgery, Western Infirmary, Glasgow.
J Clin Pathol. 1993 May;46(5):425-8. doi: 10.1136/jcp.46.5.425.
To analyse patterns of gene expression for peptide regulatory factors in patients with Dupuytren's contracture.
Tissue samples (palmer fascia) from 12 patients with Dupuytren's contracture and 12 controls were studied using the reverse transcription/polymerase chain reaction (RT/PCR) technique.
Tissue from patients with Dupuytren's contracture expressed a higher percentage of peptide regulatory factors than that of controls: interleukin-1 alpha (83% v 16%; p < 0.01); interleukin-1 beta (66% v 8%; p < 0.01); transforming growth factor beta (75% v 25%; p < 0.02); and basic fibroblast growth factor (66% v 25%; p < 0.05). Platelet derived growth factors alpha and beta were also expressed more commonly (66% v 33% and 25% v 16%, respectively), but these differences were not significant.
The increased prevalence of expression for the above mRNAs in Dupuytren's tissue is relevant as interleukin-1, basic fibroblast growth factor, and transforming growth factor beta stimulate the growth of fibroblasts and transforming growth factor beta also enhances production of collagen and other extracellular matrix proteins. Excessive local release of these peptide regulatory factors may have an important role in the pathogenesis of Dupuytren's contracture.
分析掌腱膜挛缩症患者中肽调节因子的基因表达模式。
采用逆转录/聚合酶链反应（RT/PCR）技术，对12例掌腱膜挛缩症患者和12例对照者的组织样本（掌腱膜）进行研究。
掌腱膜挛缩症患者组织中肽调节因子的表达百分比高于对照组：白细胞介素-1α（83%对16%；p<0.01）；白细胞介素-1β（66%对8%；p<0.01）；转化生长因子β（75%对25%；p<0.02）；碱性成纤维细胞生长因子（66%对25%；p<0.05）。血小板衍生生长因子α和β的表达也更常见（分别为66%对33%和25%对16%），但这些差异不显著。
掌腱膜组织中上述mRNA表达的增加具有相关性，因为白细胞介素-1、碱性成纤维细胞生长因子和转化生长因子β可刺激成纤维细胞生长，且转化生长因子β还可增强胶原蛋白和其他细胞外基质蛋白的产生。这些肽调节因子的局部过度释放可能在掌腱膜挛缩症的发病机制中起重要作用。
