Postlethwaite A E, Lachman L B, Kang A H
Arthritis Rheum. 1984 Sep;27(9):995-1001. doi: 10.1002/art.1780270905.
Human interleukin-1 (IL-1), free of contaminating lymphokines, was isolated from cultures of purified monoblasts from a patient with acute monocytic leukemia. Partially purified IL-1 (diafiltration, ultrafiltration, and isoelectric focusing) stimulated proliferation of subconfluent human fibroblasts in vitro. Further purification of IL-1 by high-resolution gel filtration- and anion exchange-high performance liquid chromatography revealed that fibroblast proliferation activity could not be separated from IL-1 activity (thymocyte proliferation), suggesting that both activities are the properties of a single molecule. Fibroblasts and thymocytes exhibited a similar sensitivity to the proliferative effects of IL-1. These findings suggest that macrophages participating in inflammatory reactions in vivo might release IL-1, which could function to expand fibroblast populations at sites of inflammatory reactions, by acting as a fibroblast growth factor.
从一名急性单核细胞白血病患者的纯化单核母细胞培养物中分离出无污染淋巴因子的人白细胞介素-1（IL-1）。部分纯化的IL-1（透析过滤、超滤和等电聚焦）在体外刺激亚汇合的人成纤维细胞增殖。通过高分辨率凝胶过滤和阴离子交换高效液相色谱对IL-1进一步纯化，结果表明成纤维细胞增殖活性与IL-1活性（胸腺细胞增殖）无法分离，这表明这两种活性是单个分子的特性。成纤维细胞和胸腺细胞对IL-1的增殖作用表现出相似的敏感性。这些发现表明，参与体内炎症反应的巨噬细胞可能释放IL-1，其可作为成纤维细胞生长因子，在炎症反应部位发挥作用以扩大成纤维细胞群体。
