González S, Díaz F, Parrado C, Pérez de Vargas I
Department of Normal and Pathological Morphology, Faculty of Medicine, University of Málaga, Spain.
J Cutan Pathol. 1993 Apr;20(2):163-7. doi: 10.1111/j.1600-0560.1993.tb00235.x.
We carried out a cytophotometric study of the DNA content of basal and suprabasal keratinocytes using involved and uninvolved histological sections of epidermis from psoriatic patients using the Feulgen technique. The reference 2C value of the DNA content was obtained from lymphocyte nuclei in a sub-epidermal infiltrate from normal skin. According to the DNA content profiles obtained for the keratinocytes of psoriatic patients, individuals were classified into three groups: Group 1, composed of individuals with a monomodal diploid profile and preferential keratinocytes with a 2C DNA content and, hence, a low proliferation activity; Group 2, consisting of patients with significantly increased 2C-4C and 4C populations; and Group 3, made up of individuals with high proportions of 4C and hyperdiploid (6C, 8C, and > 8C) keratinocytes. The results obtained in this work and the observed changes in the proliferative activity of the keratinocytes in the three groups of patients led us to suggest that the cytophotometric determination of the DNA content of basal and suprabasal keratinocytes may be useful as a prognostic criterion for the classification of psoriatic patients in terms of the extent of alteration of their proliferative epidermal activity.
我们采用福尔根技术，对银屑病患者表皮的病变和未病变组织切片中的基底角质形成细胞和基底上层角质形成细胞的DNA含量进行了细胞光度测定研究。DNA含量的参考2C值取自正常皮肤表皮下浸润淋巴细胞的细胞核。根据银屑病患者角质形成细胞的DNA含量图谱，将个体分为三组：第1组，由具有单峰二倍体图谱且优先为2C DNA含量的角质形成细胞、因而增殖活性较低的个体组成；第2组，由2C-4C和4C群体显著增加的患者组成；第3组，由4C和超二倍体（6C、8C及>8C）角质形成细胞比例较高的个体组成。本研究获得的结果以及三组患者角质形成细胞增殖活性的观察变化，使我们认为，通过细胞光度法测定基底和基底上层角质形成细胞的DNA含量，可能有助于作为根据银屑病患者增殖性表皮活性改变程度进行分类的预后标准。
