Grove G L, Anderton R L, Smith J G
J Invest Dermatol. 1976 Apr;66(4):236-8. doi: 10.1111/1523-1747.ep12482151.
Cytophotometric measurements of single-cell DNA content were used to study human epidermal cell proliferation in vivo. It was found that Feulgen-DNA profiles can be used to assess proliferative activity in involved, uninvolved, and nonpsoriatic skin. Profiles of involved psoriatic skin were bimodal as is characteristic of actively proliferating populations. This was due to the presence of cells with twice (2C--presynthetic) or four times (4C--post-synthetic) the amount of DNA of the gametes, separated by the intermediate values of cells undergoing scheduled DNA synthesis. Profiles of uninvolved psoriatic as well as nonpsoriatic epidermis were unimodal with the majority of cells containing a 2C amount of DNA incating relatively low levels of proliferative activity. The observed variations in proliferative activity of these samples are discussed in terms of two alternative models. Since radioisotopes are not required, this technique presents a useful approach to studying human epidermal proliferation in vivo.
采用细胞光度法测量单细胞DNA含量，以研究人体表皮细胞在体内的增殖情况。研究发现，福尔根DNA图谱可用于评估银屑病累及皮肤、未累及皮肤及非银屑病皮肤的增殖活性。累及的银屑病皮肤的图谱呈双峰状，这是活跃增殖群体的特征。这是由于存在DNA含量为配子两倍（2C，合成前期）或四倍（4C，合成后期）的细胞，中间值为正在进行定时DNA合成的细胞。未累及的银屑病表皮以及非银屑病表皮的图谱呈单峰状，大多数细胞含有2C量的DNA，表明增殖活性相对较低。根据两种不同的模型讨论了这些样本中观察到的增殖活性变化。由于不需要放射性同位素，该技术为研究人体表皮在体内的增殖提供了一种有用的方法。
